复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制

doi:10.11843/j.issn.0366-6964.2015.06.023

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 1055-1062.doi: 10.11843/j.issn.0366-6964.2015.06.023

复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制

韩烨晨^1#，苏晓菲^2#，王俊武¹，张娟¹，朱昱波³，程佳¹，员世宇¹，孙耀贵¹，王文魁¹，李宏全¹，段智变^1*

（1.山西农业大学动物科技学院，太谷 030801；2.山西省代县动物检疫站，代县 034200；3.河南科技学院动物科学学院，新乡 453003）

收稿日期:2015-01-13 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 段智变（1969-），女，山西祁县人，博士，教授，博士生导师，主要从事中兽医药对动物血管病变及脾胃病证研究，E-mail：duanzhibian2008@aliyun.com
作者简介:韩烨晨（1992-），女，黑龙江双鸭山人，硕士生，主要从事中药防治动物血管病变研究，E-mail：hanyechen1992@163.com；苏晓菲（1989-），女，山西忻州人，主要从事动物检疫研究，E-mail：903451453@qq.com。韩烨晨和苏晓菲同为第一作者
基金资助:
山西省科技攻关项目（20140311022-1）；山西农业大学横向科技项目（2013HX28）

The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO，ET-1 in Lung of Broiler with Ascites Syndrome

HAN Ye-chen^1# ，SU Xiao-fei^2# ，WANG Jun-wu¹，ZHANG Juan¹，ZHU Yu-bo³，CHENG Jia¹，YUN Shi-yu¹，SUN Yao-gui¹，WANG Wen-kui¹，LI Hong-quan¹，DUAN Zhi-bian^1*

(1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China；2.Animal Quarantine Station of Daixian of Shanxi，Daixian 034200，China；3.College of Animal Scinece，Henan Institute of Science and Technology，Xinxiang 453003，China)

Received:2015-01-13 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

为研究腹水综合征（AS）肉鸡肺组织NO、ET-1及相关指标变化和复方中药对其的防治作用，将316羽14日龄罗斯肉鸡随机分为空白组（N组，常温，常规饲养）、模型组（C组，9~11 ℃，添加3%猪油和4%鱼粉的高能饲料，0.12% Na⁺饮水饲养）和复方中药高（T1）、中（T2）、低（T3）剂量组（饲养同C组，每天于饮水中分别给予2、1、0.5 mL•kg^-1复方中药）以及L-Arg组（L组，饲养同C组，每天于饲料中添加1% L-Arg）。结果表明：与N组相比，35和45日龄C组肉鸡出现典型临床症状，腹水发病率、腹水心脏指数和肺组织NO及ET-1含量、iNOS活性、ETAR mRNA相对表达量显著增加（P<0.01或P<0.05），肺组织cNOS活性和NO/ET-1显著降低（P<0.01）；与C组相比，T1、T2和L组均能改善临床症状及上述指标（P<0.01或P<0.05）。肉鸡肺组织NO及ET-1含量、ETAR mRNA相对表达量和iNOS活性升高，cNOS活性降低，导致NO、ET-1失衡，参与了AS的发生；气血双补、行气利水的复方中药能有效防治AS。

Abstract:

The objective of this study was to investigate the changes of the contents of nitric oxide (NO) and endothelin-1 (ET-1)，and the related items in lung of broilers with ascites syndrome (AS) and the prevention and treatment of the compound recipe of the traditional Chinese medicine on AS.A total of 316 14-day-old Ross broilers were randomly divided into normal group (N，bred with a standard diet at room temperature)，control group (C，bred with high-energy diet derived from standard diet added with 3% lard and 4% fish meal and 0.12% Na⁺ in drinking water at 9-11 ℃ to induce AS)，compound recipe of the traditional Chinese medicine groups (T1，T2，T3，bred in the same way as group C except a dose of 2，1，0.5 mL•kg^-1 bw of the traditional Chinese medicine everyday added in drinking water，respectively)，and L-arginine group (L，bred in the same way as group C except 1% L-arginine added in diet).The results showed that the broilers in group C presented the typical clinical symptoms of AS at 35 and 45 days of age.Compared with group N，the incidence of AS，ascites heart index (AHI)，the contents of NO and ET-1，iNOS activity and the relative expression amount of ETAR mRNA in lung of broilers at 35 and 45 days of age in group C were increased significantly (P<0.01 or P<0.05)，and cNOS activity and the ratio of NO to ET-1 were decreased significantly (P<0.01) in group C.The clinical symptoms and items mentioned above were all improved in groups of L，T1 and T2 compared with group C (P<0.01 or P<0.05).The study showed that the increases of NO and ET-1 content，ETAR mRNA relative expression amount and iNOS activity and the decrease of cNOS activity in lung of broiler induced the unbalance of NO and ET-1 which participated in the pathogenesis of AS.The studied compound recipe of the traditional Chinese medicine with the role of tonifying qi-blood and activating qi to excrete water can prevent and treat AS of broiler effectively.

中图分类号:

S853.33

韩烨晨，苏晓菲，王俊武，张娟，朱昱波，程佳，员世宇，孙耀贵，王文魁，李宏全，段智变. 复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制[J]. 畜牧兽医学报, 2015, 46(6): 1055-1062.

HAN Ye-chen，SU Xiao-fei，WANG Jun-wu，ZHANG Juan，ZHU Yu-bo，CHENG Jia，YUN Shi-yu，SUN Yao-gui，WANG Wen-kui，LI Hong-quan，DUAN Zhi-bian. The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO，ET-1 in Lung of Broiler with Ascites Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1055-1062.

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复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制

The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO，ET-1 in Lung of Broiler with Ascites Syndrome

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